Microbiology Lab

Unknown Final report
1. Introduction:
The purpose of this lab was to spot two unfathomable bacterium cultures using various derivative bear witnesss.   The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specialized biochemical characteristics.   Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was use in a way to help recognize those specifics and identify the unknown cultures.   The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and saccharose fermentation, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, indole, urease, DNase, and coagulase.  
The tests performed on the unknown bacteria cultures were all used to determine the identity of the bacteria.   Each of the tests performed provided most key information about the bacteria in head word and how it functions.   All of the tests were performed on every culture, however, as some of the tests were used only for gram (+) or gram (-) bacteria, while others were nevertheless more specific and identified only for cocci bacteria.   The tests performed and what constitutes a positive and negative test are as follows.

2.



tryout Result of all organism similar to my unknown
See following(a) pages

3. Flow chart
See page with flow chart

4. Test Result
Oxidase
This testing ground test is based on detecting the production of the enzyme cytochrome oxidase by Gram-negative bacteria if no color change to purple or blue is evident at 30 seconds-1 slight then the result is positive. It is important that the test is read by one minute to ensure accurate results (avoid trumped-up(prenominal) negatives and false positives).  Unknown 12 resulted in a negative test with no color change. These were similar results seen in Eschericia coli, Proteus vulgaris, genus Serratia marcescens, and Enterobacter aerogenes. This test helped to rule out two of the six unknown...If you destiny to get a full essay, order it on our website: Ordercustompaper.com



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